Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: Reagents are loaded in a 96-well plate, and a programming script is used to control their flow into a single chamber or into four chambers containing an ElectraSense microarray. Current or voltage can be applied to single or groups of electrodes for Ppy deposition, and current can be read from a single electrode or groups of electrodes for ECD.

Article Snippet: Hybridization to probes can be detected using cyanine (Cy) dyes and fluorescent scanners or, alternatively, using horseradish peroxidase (HRP) and enzyme-enhanced electrochemical detection (ECD) on CombiMatrix's microarray readers.

Techniques: Microarray

Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: A) Photomicrograph of an ElectraSense microarray under epi illumination and showing the deposition of polypyrrole with adsorbed murine IgG on a 5×5 set of electrodes. B) Fluorescence scanned image of the microarray treated with Cy5-labeled goat anti mouse IgG and showing the presence of murine Ab on the electrodes.

Article Snippet: Hybridization to probes can be detected using cyanine (Cy) dyes and fluorescent scanners or, alternatively, using horseradish peroxidase (HRP) and enzyme-enhanced electrochemical detection (ECD) on CombiMatrix's microarray readers.

Techniques: Microarray, Fluorescence, Labeling

Journal: PLoS ONE

Article Title: Targeted Deposition of Antibodies on a Multiplex CMOS Microarray and Optimization of a Sensitive Immunoassay Using Electrochemical Detection

doi: 10.1371/journal.pone.0009781

Figure Lengend Snippet: A ) Three concentrations (0.003, 0.01, and 0.03 pg/ml) were incubated on a microarray with either anti-SEB MAb (•-•) or casein (▪-▪) adsorbed onto Ppy that was deposited at 40 nA for 1 s. SEB binding was detected using biotinylated rabbit anti-SEB Ab and SA-HRP. Background (0 pg/ml SEB) was 1147±283. B ) Different concentrations of SEB were incubated on a microtiter plate with anti-SEB MAb adsorbed onto the surface of each well. SEB binding was detected using biotinylated rabbit anti-SEB Ab and SA-HRP. Background (0 pg/ml SEB) was OD 450–570 2.939±.026.

Article Snippet: Hybridization to probes can be detected using cyanine (Cy) dyes and fluorescent scanners or, alternatively, using horseradish peroxidase (HRP) and enzyme-enhanced electrochemical detection (ECD) on CombiMatrix's microarray readers.

Techniques: Incubation, Microarray, Binding Assay